The PEF family proteins sorcin and grancalcin interact in vivo and in vitro.
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Hansen C, Tarabykina S, la Cour JM, Lollike K, Berchtold MW
The PEF family proteins sorcin and grancalcin interact in vivo and in vitro.
FEBS Lett. 2003 Jun 19;545(2-3):151-4.
- PubMed ID
- 12804766 [ View in PubMed]
- Abstract
The penta-EF hand (PEF) family of calcium binding proteins includes grancalcin, peflin, sorcin, calpain large and small subunits as well as ALG-2. Systematic testing of the heterodimerization abilities of the PEF proteins using the yeast two-hybrid and glutathione S-transferase pull-down assays revealed the new finding that grancalcin interacts strongly with sorcin. In addition, sorcin and grancalcin can be co-immunoprecipitated from lysates of human umbilical vein endothelial cells. Our results indicate that heterodimerization, in addition to differential interactions with target proteins, might be a way to regulate and fine tune processes mediated by calcium binding proteins of the penta-EF hand type.