Qualitative and quantitative difference in mutation induction between carbon- and neon-ion beams in normal human cells.

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Suzuki M, Tsuruoka C, Kanai T, Kato T, Yatagai F, Watanabe M

Qualitative and quantitative difference in mutation induction between carbon- and neon-ion beams in normal human cells.

Biol Sci Space. 2003 Dec;17(4):302-6.

PubMed ID

15136753 [ View in PubMed

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Abstract

We investigated the difference in cell-killing effect and mutation induction between carbon- and neon-ion beams in normal human cells. Carbon- and neon-ion beams were accelerated by the Riken Ring Cyclotron (RRC) at the Institute of Physical and Chemical Research in Japan. Cell-killing effect was measured as the reproductive cell death using the colony formation assay. Mutation induction at the HPRT locus was detected to measure 6-thioguanine-resistant clones. The mutation spectrum of the deletion pattern of exons of induced mutants was analyzed using the multiplex polymerase chain reaction (PCR). Cell-killing effect was almost the same between carbon- and neon-ion beams with similar linear energy transfer (LET) values, while there observed a large difference in mutation frequency. Furthermore, in the case of neon-ion beams 60% of mutants showed total deletions and 35-40% showed partial deletions, while 95-100% of carbon-ion induced mutants showed total deletions. The results suggest that different ion species may cause qualitative and quantitative difference in mutation induction even if the LET values are similar.

DrugBank Data that Cites this Article

Drug Enzymes

Drug	Enzyme	Kind	Organism	Pharmacological Action	Actions
Tioguanine	Hypoxanthine-guanine phosphoribosyltransferase	Protein	Humans	Unknown	Substrate	Details