Inhibition by mepacrine and amylase secretion from intact and permeabilized rat pancreatic acini.

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Grosfils K, Gomez F, Dehaye JP

Inhibition by mepacrine and amylase secretion from intact and permeabilized rat pancreatic acini.

Biochem Biophys Res Commun. 1992 Apr 15;184(1):408-13.

PubMed ID

1373616 [ View in PubMed

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Abstract

In intact rat pancreatic acini, the phospholipase A2 inhibitor mepacrine did not affect basal amylase release but dose-dependently inhibited the carbachol (IC50 65 microM) and CCK-8 (IC50 210 microM)-stimulated amylase release. In permeabilized acini, mepacrine shifted the dose-response curve for calcium to the right by a factor 2 and inhibited the release of amylase stimulated by GTPrS. From these results we conclude that carbachol, CCK-8 and GTPrS probably activate a phospholipase A2 closely coupled to exocytosis.

DrugBank Data that Cites this Article

Drug Enzymes

Drug	Enzyme	Kind	Organism	Pharmacological Action	Actions
Carbamoylcholine	Cytosolic phospholipase A2	Protein	Humans	Unknown	Inducer	Details