Metronidazole induces programmed cell death in the protozoan parasite Blastocystis hominis.

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Nasirudeen AM, Hian YE, Singh M, Tan KS

Metronidazole induces programmed cell death in the protozoan parasite Blastocystis hominis.

Microbiology. 2004 Jan;150(Pt 1):33-43. doi: 10.1099/mic.0.26496-0.

PubMed ID

14702395 [ View in PubMed

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Abstract

Previous studies by the authors have shown that the protozoan parasite Blastocystis hominis succumbed to a cytotoxic monoclonal antibody with a number of cellular and biochemical features characteristic of apoptosis in higher eukaryotes. The present study reports that apoptosis-like features are also observed in growing cultures of axenic B. hominis upon exposure to metronidazole, a drug commonly used for the treatment of blastocystosis. Upon treatment with the drug, B. hominis cells displayed key morphological and biochemical features of programmed cell death (PCD), viz. nuclear condensation and nicked DNA in nucleus, reduced cytoplasmic volume, externalization of phosphatidylserine and maintenance of plasma membrane integrity with increasing permeability. This present study also supports the authors' previously postulated novel function for the B. hominis central vacuole in PCD; it acts as a repository where apoptotic bodies are stored before being released into the extracellular space. The implications and possible roles of PCD in B. hominis are discussed.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Metronidazole	Protozoal DNA	Group		Unknown	Inhibitor	Details