Echinacoside rescues the SHSY5Y neuronal cells from TNFalpha-induced apoptosis.

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Deng M, Zhao JY, Tu PF, Jiang Y, Li ZB, Wang YH

Echinacoside rescues the SHSY5Y neuronal cells from TNFalpha-induced apoptosis.

Eur J Pharmacol. 2004 Nov 28;505(1-3):11-8. doi: 10.1016/j.ejphar.2004.09.059.

PubMed ID
15556132 [ View in PubMed
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Abstract

We investigated the neuroprotective effect of echinacoside, one of the phenylethanoids isolated from the stems of Cistanches salsa, a Chinese herbal medicine, on tumor necrosis factor-alpha (TNFalpha)-induced apoptosis in human neuroblastoma (SHSY5Y) cells. Treatment of cultured SHSY5Y cells with TNFalpha 100 ng ml(-1) for 36 h stimulated apoptosis, as demonstrated by typical morphological changes, cell viability, DNA laddering, annexin-V binding, intracellular reactive oxygen species, mitochondrial membrane potential and caspase-3 activity. However, simultaneous treatment with echinacoside (1, 10 or 100 microg ml(-1)) attenuated the TNFalpha-mediated apoptosis. The antiapoptotic action of echinacoside was partially dependent on antioxidative stress effects, maintenance of mitochondria function, inhibition of caspase-3 activity and was also associated with increasing the expression of the antiapoptotic protein Bcl2. Thus, echinacoside has the neuroprotective capacity to antagonize TNFalpha-induced apoptosis in SHSY5Y cells and may be useful in treating some neurodegenerative diseases.

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