In vitro amiodarone protein binding and its interaction with warfarin.

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Neyroz P, Bonati M

In vitro amiodarone protein binding and its interaction with warfarin.

Experientia. 1985 Mar 15;41(3):361-3. doi: 10.1007/bf02004505.

PubMed ID

3972082 [ View in PubMed

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Abstract

The binding of amiodarone to human plasma protein and to bovine serum albumin was studied by three different methods, ultracentrifugation, equilibrium dialysis and fluorescence spectroscopy. The fraction of amiodarone bound to plasma protein amounted to 96.3%. The changes in the binding properties of 1-anilino-naphthalene-8-sulfonate for bovine serum albumin using warfarin and amiodarone as independent inhibitors were analyzed in terms of binding site specificity. The findings indicated that amiodarone and warfarin have two different binding sites on bovine serum albumin, so a noncompetitive inhibition mechanism was indicated. On the basis of our data we cannot exclude other mechanisms of interaction besides direct displacement of one drug by another; nevertheless, metabolite interference between amiodarone and coagulation cofactors may better explain the enhancement of warfarin's pharmacological action in association with amiodarone.

DrugBank Data that Cites this Article

Drug Carriers

Drug	Carrier	Kind	Organism	Pharmacological Action	Actions
Amiodarone	Serum albumin	Protein	Humans	Unknown	Binder	Details