Hepatic uptake and biliary excretion of manganese in the little skate, Leucoraja erinacea.

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Madejczyk MS, Boyer JL, Ballatori N

Hepatic uptake and biliary excretion of manganese in the little skate, Leucoraja erinacea.

Comp Biochem Physiol C Toxicol Pharmacol. 2009 May;149(4):566-71. doi: 10.1016/j.cbpc.2008.12.009. Epub 2008 Dec 24.

PubMed ID

19141331 [ View in PubMed

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Abstract

The liver is a major organ involved in regulating whole body manganese (Mn) homeostasis; however, the mechanisms of Mn transport across the hepatocyte basolateral and canalicular membranes remain poorly defined. To gain insight into these transport steps, the present study measured hepatic uptake and biliary excretion of Mn in an evolutionarily primitive marine vertebrate, the elasmobranch Leucoraja erinacea, the little skate. Mn was rapidly removed from the recirculating perfusate of isolated perfused skate livers in a dose-dependent fashion; however, only a small fraction was released into bile (<2% in 6 h). Mn was also rapidly taken up by freshly isolated skate hepatocytes in culture. Mn uptake was inhibited by a variety of divalent metals, but not by cesium. Analysis of the concentration-dependence of Mn uptake revealed of two components, with apparent K(m) values 1.1+/-0.1 microM and 112+/-29 microM. The K(m) value for the high-affinity component was similar to the measured skate blood Mn concentration, 1.9+/-0.5 microM. Mn uptake was reduced by nearly half when bicarbonate was removed from the culture medium, but was unaffected by a change in pH from 6.5 to 8.5, or by substitution of Na with Li or K. Mn efflux from the hepatocytes was also rapid, and was inhibited when cells were treated with 0.5 mM 2,4-dinitrophenol to deplete ATP levels. These data indicate that skate liver has efficient mechanisms for removing Mn from the sinusoidal circulation, whereas overall biliary excretion is low and appears to be mediated in part by an ATP-sensitive mechanism.

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Drug Transporters

Drug	Transporter	Kind	Organism	Pharmacological Action	Actions
Mangafodipir	Natural resistance-associated macrophage protein 2	Protein	Humans	Unknown	Substrate	Details