Recombinant human estrogen, androgen and progesterone receptors for detection of potential endocrine disruptors.

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Scippo ML, Argiris C, Van De Weerdt C, Muller M, Willemsen P, Martial J, Maghuin-Rogister G

Recombinant human estrogen, androgen and progesterone receptors for detection of potential endocrine disruptors.

Anal Bioanal Chem. 2004 Feb;378(3):664-9. Epub 2003 Oct 25.

PubMed ID
14579009 [ View in PubMed
]
Abstract

This work reports the binding capacity of various chemicals (so-called endocrine disruptors) to recombinant human steroid receptors (hERalpha, hPR and hAR). The tested chemicals are organochlorine insecticides (DDT and its metabolites, methoxychlor, aldrin, dieldrin, chlordecone, lindane, trichlorobenzene), estrogenic insecticides (endosulfan, toxaphene, nonachlor), herbicides (alachlor and atrazine), fungicides (benomyl and vinclozolin), industrial chemicals (nonylphenol, bisphenol A, diphenylphtalate), antioxidants (butylated hydroxyanisol) and some phytoestrogens. Except for phytoestrogens, most of the tested chemicals (DDT and its metabolites, aldrin, alpha- and beta-endosulfan, toxaphen, trans-nonachlor) show higher affinities for hPR than for hERalpha, indicating that the interaction with the progesterone receptor could contribute to the endocrine-disrupting effects imputed to these chemicals. We propose to use binding assays using recombinant human steroid receptors as screening tools for the detection of endocrine disruptors in various samples.

DrugBank Data that Cites this Article

Drug Targets
DrugTargetKindOrganismPharmacological ActionActions
LindaneEstrogen receptor alphaProteinHumans
Unknown
Not AvailableDetails
LindaneProgesterone receptorProteinHumans
Unknown
Not AvailableDetails
MitotaneEstrogen receptor alphaProteinHumans
Unknown
Not AvailableDetails
MitotaneProgesterone receptorProteinHumans
Unknown
Not AvailableDetails
Phthalic AcidEstrogen receptor alphaProteinHumans
Unknown
Not AvailableDetails
Phthalic AcidProgesterone receptorProteinHumans
Unknown
Not AvailableDetails