Expression of vanilloid receptor subtype 1 in cutaneous sensory nerve fibers, mast cells, and epithelial cells of appendage structures.
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Stander S, Moormann C, Schumacher M, Buddenkotte J, Artuc M, Shpacovitch V, Brzoska T, Lippert U, Henz BM, Luger TA, Metze D, Steinhoff M
Expression of vanilloid receptor subtype 1 in cutaneous sensory nerve fibers, mast cells, and epithelial cells of appendage structures.
Exp Dermatol. 2004 Mar;13(3):129-39.
- PubMed ID
- 14987252 [ View in PubMed]
- Abstract
The vanilloid receptor subtype 1 (VR1)/(TRPV1), binding capsaicin, is a non-selective cation channel that recently has been shown in human keratinocytes in vitro and in vivo. However, a description of VR1 localization in other cutaneous compartments in particular cutaneous nerve fibers is still lacking. We therefore investigated VR1 immunoreactivity as well as mRNA and protein expression in a series (n = 26) of normal (n = 7), diseased (n = 13) [prurigo nodularis (PN) (n = 10), generalized pruritus (n = 1), and mastocytosis (n = 2)], and capsaicin-treated human skin (n = 6). VR1 immunoreactivity could be observed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands. Upon reverse transcriptase-polymerase chain reaction and Western blot analysis, VR1 was detected in mast cells and keratinocytes from human skin. In pruritic skin of PN, VR1 expression was highly increased in epidermal keratinocytes and nerve fibers, which was normalized after capsaicin application. During capsaicin therapy, a reduction of neuropeptides (substance P, calcitonin gene-related peptide) was observed. After cessation of capsaicin therapy, neuropeptides re-accumulated in skin nerves. In conclusion, VR1 is widely distributed in the skin, suggesting a major role for this receptor, e.g. in nociception and neurogenic inflammation.
DrugBank Data that Cites this Article
- Polypeptides
Name UniProt ID Transient receptor potential cation channel subfamily V member 1 Q8NER1 Details - Pharmaco-transcriptomics
Drug Drug Groups Gene Gene ID Change Interaction Chromosome Capsaicin Approved TAC1 6863 downregulated Capsaicin results in decreased expression of TAC1 mRNA 7q21.3 Capsaicin Approved TRPV1 7442 downregulated Capsaicin results in decreased expression of TRPV1 mRNA 17p13.2 - Pharmaco-proteomics
Drug Drug Groups Gene Gene ID Change Interaction Chromosome Capsaicin Approved TAC1 6863 decreased Capsaicin results in decreased expression of TAC1 protein 7q21.3 Capsaicin Approved TRPV1 7442 decreased Capsaicin results in decreased expression of TRPV1 protein 17p13.2