Molecular cloning and chromosomal mapping of CCND genes encoding human D-type cyclins.
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Xiong Y, Menninger J, Beach D, Ward DC
Molecular cloning and chromosomal mapping of CCND genes encoding human D-type cyclins.
Genomics. 1992 Jul;13(3):575-84. doi: 10.1016/0888-7543(92)90127-e.
- PubMed ID
- 1386336 [ View in PubMed]
- Abstract
A human D-type cyclin gene (CCND1/cyclin D1/PRAD1) was previously isolated by virtue of its ability to complement a triple G1 cyclin (Cln) deficiency of Saccharomyces cerevisiae and was also identified as a candidate BCL1 oncogene. We now report the molecular cloning of two additional human D-type cyclin genes, CCND2 (cyclin D2) and CCND3 (cyclin D3). All three human D-type cyclin genes encode small (33-34 kDa) proteins that share an average of 57% identity over the entire coding region and 78% in the cyclin box. The D-type cyclins are most closely related to cyclin A (39% identity) and cyclin E (36%), followed by cyclin B (29%) and cyclin C (21%). Isolation and characterization of genomic clones revealed two pseudogenes corresponding to CCND2 and CCND3, respectively. All three cyclin D genes are interrupted by an intron at the same position. CCND2 has been mapped to chromosome 12p13, and CCND3 has been mapped to chromosome 6p21.