Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein.
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Ma WJ, Cheng S, Campbell C, Wright A, Furneaux H
Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein.
J Biol Chem. 1996 Apr 5;271(14):8144-51. doi: 10.1074/jbc.271.14.8144.
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- 8626503 [ View in PubMed]
- Abstract
The neuronal-specific Elav-like proteins (HuD, Hel-N, and HuC) contain three RNP-type concensus motifs and bind to AU-rich elements. We have identified and cloned a fourth member of this family (HuR) that is expressed in a wide variety of cell types. The purified recombinant protein binds avidly to the AU-rich element in c-fos and interleukin-3 mRNAs. In the case of the c-fos AU-rich element, HuR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Mutational analysis has shown that all three AU motifs are required for maximal binding.