Structure and sequence analysis of the human activin beta A subunit gene.
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Tanimoto K, Handa S, Ueno N, Murakami K, Fukamizu A
Structure and sequence analysis of the human activin beta A subunit gene.
DNA Seq. 1991;2(2):103-10. doi: 10.3109/10425179109039678.
- PubMed ID
- 1777673 [ View in PubMed]
- Abstract
The cloned genomic DNA containing the human activin beta A subunit gene were analyzed by restriction endonuclease mapping, Southern blotting and DNA sequencing. The activin gene is composed of two exons interrupted by the 9-kb intron. The TATA, CCAAT and CT-stretch sequences were found in the 5'-flanking region of the gene. An intronic sequence contained SV40 enhancer core element in the vicinity of the exon 1. In the 3'-flanking region, we identified eight consensus polyadenylation sequences, five ATTTA motifs, CA element consisting of (CA)14, AP-1 binding site and two SV40 enhancer core elements. A dot matrix analysis revealed the high degree of conservation between the human and rat sequences within the 3'-flanking region, suggesting a possible functional significance.