In vivo regulation of the uvrA gene: role of the "-10" and "-35" promoter regions.
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Backendorf C, Brandsma JA, Kartasova T, van de Putte P
In vivo regulation of the uvrA gene: role of the "-10" and "-35" promoter regions.
Nucleic Acids Res. 1983 Sep 10;11(17):5795-810. doi: 10.1093/nar/11.17.5795.
- PubMed ID
- 6310514 [ View in PubMed]
- Abstract
The effect of increasing deletions in the uvrA promoter region on the transcriptional efficiency was quantitatively analysed by fusion to the galK structural gene. A physical analysis of uvrA messenger RNA synthesis from the different deletion plasmids was performed using the S1 mapping technique. Both methods indicate that the uvrA "-10" promoter sequence is sufficient to trigger uvrA transcription. Although not essential, the "-35" region, which is overlapping with the LexA binding site, is shown to have an enhancing function, as the exposure of this region after SOS induction results in a 3- to 4-fold increase in uvrA transcription. A model is presented which accounts both for the observed basal and induced expression of the uvrA gene on a molecular level.