Expression of active human factor VIII from recombinant DNA clones.
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Wood WI, Capon DJ, Simonsen CC, Eaton DL, Gitschier J, Keyt B, Seeburg PH, Smith DH, Hollingshead P, Wion KL, Delwart E, Tuddenham EG, Vehar GA, Lawn RM
Expression of active human factor VIII from recombinant DNA clones.
Nature. 1984 Nov 22-28;312(5992):330-7.
- PubMed ID
- 6438526 [ View in PubMed]
- Abstract
DNA clones encoding the complete 2,351 amino acid sequence for human factor VIII have been isolated and used to produce biologically active factor VIII in cultured mammalian cells. The recombinant protein corrects the clotting time of plasma from haemophiliacs and has many of the biochemical and immunological characteristics of serum-derived factor VIII.