Neuronal ion channels as the target sites of insecticides.

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Narahashi T

Neuronal ion channels as the target sites of insecticides.

Pharmacol Toxicol. 1996 Jul;79(1):1-14.

PubMed ID

8841090 [ View in PubMed

]

Abstract

Certain types of neuronal ions channels have been demonstrated to be the major target sites of insecticides. The insecticide-channel interactions that have been studied most extensively are pyrethroid actions on the voltage-gated sodium channel and cyclodiene/lindane actions on the GABAA receptor chloride channel complex. With the exception of organophosphate and carbamate insecticides which inhibit acetylcholinesterases, most insecticide commercially developed act on the sodium channel and the GABA system. Pyrethroids show the kinetics of both activation and inactivation gates of sodium channels resulting in prolonged openings of individual channels. This causes membrane depolarization, repetitive discharges and synaptic disturbances leading to hyperexcitatory symptoms of poisoning in animals. Only a very small fraction (approximately 1%) of sodium channel population is required to be modified by pyrethroids to produce severe hyperexcitatory symptoms. This toxicity amplification theory applies to pharmacological and toxicological action of other drugs that go through a threshold phenomenon. Selective toxicity of pyrethroids between invertebrates and mammals can be explained based largely on the responses of sodium channels and partly on metabolic degradation. The pyrethroid-sodium channel interaction is also supported by Na+ uptake and batrachotoxin binding experiments. Cyclodienes and lindane exert a dual action on the GABAA system, the initial transient stimulation being followed by a suppression. The stimulation requires the presence of the gamma 2 subunit. The suppression of the GABA system is also documented by Cl- flux and ligand binding experiments. It appears that the sodium channel and the GABA system merit continuing efforts for development of newer and better insecticides. Nitromethylene heterocycles including imidacloprid act on nicotinic acetylcholine receptors. Insect receptors are more sensitive to these compounds than mammalian receptors. Single-channel analyses of the nicotinic acetylcholine receptor of PC12 cells have shown that imidacloprid increases the activity of subconductance state currents and decreases that of main conductance state currents. This may explain the imidacloprid suppression of acetylcholine responses.

DrugBank Data that Cites this Article

Drugs

Bioallethrin

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Bioallethrin	Sodium channel protein	Protein	Pediculus humanus	Yes	Modulator	Details
Bioallethrin	Voltage-gated sodium channel alpha subunit (Protein Group)	Protein group	Humans	Unknown	Modulator	Details
Lindane	Gamma-aminobutyric acid receptor subunit beta-1	Protein	Humans	Yes	Antagonist	Details