Cloning of a human cDNA encoding a protein with high homology to yeast methionyl-tRNA synthetase.
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Lage H, Dietel M
Cloning of a human cDNA encoding a protein with high homology to yeast methionyl-tRNA synthetase.
Gene. 1996 Oct 31;178(1-2):187-9.
- PubMed ID
- 8921912 [ View in PubMed]
- Abstract
A composite 2779-bp cDNA that encodes human cytoplasmic methionyl-tRNA synthetase (MetRS) has been constructed from partial cDNA clones derived from the gastric carcinoma cell line EPG85-257RNOV and the nucleotide sequence has been determined. The open reading frame (ORF) encodes a 900 amino acid (aa) protein with a predicted molecular mass of 101 kDa. Northern blotting analysis of total RNA extracted from human gastric carcinoma cells demonstrated a single band with a mobility corresponding to a size of 3.0 kb.