Active site of human liver aldehyde dehydrogenase.

Article Details


Abriola DP, Fields R, Stein S, MacKerell AD Jr, Pietruszko R

Active site of human liver aldehyde dehydrogenase.

Biochemistry. 1987 Sep 8;26(18):5679-84.

PubMed ID
3676276 [ View in PubMed

Bromoacetophenone (2-bromo-1-phenylethanone) functions as an affinity reagent for human aldehyde dehydrogenase (EC and has been found specifically to label a unique tryptic peptide in the enzyme. Amino-terminal sequence analysis of the labeled peptide after purification by two different procedures revealed the following sequence: Val-Thr-Leu-Glu-Leu-Gly-Gly-Lys. Radioactivity was found to be associated with the glutamate residue, which was identified as Glu-268 by reference to the known amino acid sequence. This paper constitutes the first identification of an active site of aldehyde dehydrogenase.

DrugBank Data that Cites this Article

NameUniProt ID
Retinal dehydrogenase 1P00352Details