Complete amino acid sequence of the human progesterone receptor deduced from cloned cDNA.

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Citation

Misrahi M, Atger M, d'Auriol L, Loosfelt H, Meriel C, Fridlansky F, Guiochon-Mantel A, Galibert F, Milgrom E

Complete amino acid sequence of the human progesterone receptor deduced from cloned cDNA.

Biochem Biophys Res Commun. 1987 Mar 13;143(2):740-8.

PubMed ID
3551956 [ View in PubMed
]
Abstract

A lambda gt10 library containing DNAs complementary to messenger RNAs from human breast cancer T47-D cells was constructed and screened with a cDNA probe encoding the rabbit progesterone receptor. Four overlapping clones have been sequenced. The open reading frame corresponds to a protein of 933 amino acids with a molecular weight of 98,868 Da. The cysteine rich basic region supposed to be involved in DNA binding is completely homologous in the human and rabbit receptors, whereas the C-terminal end, where hormone binding is thought to take place, differs by a single amino acid change. The human progesterone receptor is characterized, as is the rabbit receptor, by the very high proline content of its N-terminal region. When mRNAs from either human breast cancer cell lines T47-D and MCF-7 or from normal human uterus tissue were blotted and probed with the cloned cDNA, four main bands were observed (5100, 4300, 3700, and 2900 nucleotides).

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Progesterone receptorP06401Details