Human cysteine dioxygenase type I: primary structure derived from base sequencing of cDNA.
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McCann KP, Akbari MT, Williams AC, Ramsden DB
Human cysteine dioxygenase type I: primary structure derived from base sequencing of cDNA.
Biochim Biophys Acta. 1994 Nov 16;1209(1):107-10.
- PubMed ID
- 7524679 [ View in PubMed]
- Abstract
The base sequence of cDNA encoding the complete human liver cysteine dioxygenase type I (CDO-I; EC 1.13.11.20) message, and the derived amino-acid sequence are reported. CDO-I is encoded on a single mRNA species (approx. 1.5 kb). Human CDO-I clones were identified by screening a liver cDNA library, and inserts were isolated and sequenced. In addition, human liver total RNA was reverse-transcribed and CDO-I cDNA amplified by PCR using a modified poly-T primer and specific CDO-I primers to give a second source of sequencing template. The CDO-I message encodes a 200 amino-acid residue protein, the sequence of which has greater than 90% homology with the equivalent rat enzyme. The message was not expressed in a human hepatoma cell line (Hep G2).