Molecular cloning of the human T-lymphocyte surface CD2 (T11) antigen.

Article Details

Citation

Sewell WA, Brown MH, Dunne J, Owen MJ, Crumpton MJ

Molecular cloning of the human T-lymphocyte surface CD2 (T11) antigen.

Proc Natl Acad Sci U S A. 1986 Nov;83(22):8718-22.

PubMed ID
3490670 [ View in PubMed
]
Abstract

CD2 (T11, sheep erythrocyte receptor) is a surface antigen of the human T-lymphocyte lineage. cDNA clones encoding CD2 have been isolated by using the purified, denatured CD2 to raise a rat antiserum. Positive clones were recognized in a phage lambda gt11 expression library prepared from the human leukemia T-cell line J6. The DNA sequence contained an open reading frame encoding 360 amino acids. The N-terminal 24 amino acids were characteristic of a signal peptide and were followed by a region that matched all 25 residues of the CD2 N terminus previously determined by amino acid sequencing. The predicted amino acid sequence is consistent with that of a transmembrane glycoprotein containing three potential N-glycosylation sites on the N-terminal side of a 26-amino acid hydrophobic segment. There is a large cytoplasmic domain of 125 amino acids that is rich in proline and in basic residues. RNA blot-hybridization analysis demonstrated hybridization only in those T cells that were positive for surface CD2 antigen. There are limited regions of sequence similarity to members of the immunoglobulin supergene family.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
T-cell surface antigen CD2P06729Details