DNA sequence of the E. coli gyrB gene: application of a new sequencing strategy.
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Adachi T, Mizuuchi M, Robinson EA, Appella E, O'Dea MH, Gellert M, Mizuuchi K
DNA sequence of the E. coli gyrB gene: application of a new sequencing strategy.
Nucleic Acids Res. 1987 Jan 26;15(2):771-84.
- PubMed ID
- 3029692 [ View in PubMed]
- Abstract
We have determined the sequence of the E. coli gyrB gene, using a new sequencing approach in which transposition from a mini-Mu plasmid into the DNA provides random start points for dideoxynucleotide sequence analysis. The gyrB sequence corresponds to a protein 804 amino acids long; a previously isolated protein fragment with partial enzymatic activity has been identified as the C-terminal half-molecule. A plausible terminator of gyrB transcription is located just beyond the structural gene.