Extracellular signal-regulated kinases in T cells: characterization of human ERK1 and ERK2 cDNAs.
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Owaki H, Makar R, Boulton TG, Cobb MH, Geppert TD
Extracellular signal-regulated kinases in T cells: characterization of human ERK1 and ERK2 cDNAs.
Biochem Biophys Res Commun. 1992 Feb 14;182(3):1416-22.
- PubMed ID
- 1540184 [ View in PubMed]
- Abstract
Extracellular signal-regulated kinases 1 and 2 are growth factor-sensitive serine/threonine kinases. cDNAs for both human kinases were isolated and sequenced. The nucleic acid and deduced protein sequences of human extracellular signal-regulated kinase 1 were 88% and 96% identical, respectively, to the homologous rat sequences. The nucleic acid and deduced protein sequences of human extracellular signal-regulated kinase 2 were 90% and 98% identical, respectively, to the corresponding rat sequences. A human extracellular signal-regulated kinase 2 specific probe was used to demonstrate that the mRNA for this kinase was present in T cells and did not change with activation. The deduced protein sequences of both human kinases were greater than 95% identical to two Xenopus kinase sequences, indicating that these enzymes are highly conserved across species.