Sequencing of a urinary stone protein, identical to alpha-one antitrypsin, which lacks 22 amino acids.
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Umekawa T, Kohri K, Amasaki N, Yamate T, Yoshida K, Yamamoto K, Suzuki Y, Sinohara H, Kurita T
Sequencing of a urinary stone protein, identical to alpha-one antitrypsin, which lacks 22 amino acids.
Biochem Biophys Res Commun. 1993 Jun 30;193(3):1049-53.
- PubMed ID
- 8323530 [ View in PubMed]
- Abstract
We have extracted and purified proteins from calcium containing urinary stones with 4 M guanidine chloride and sequenced the amino acids of the proteins. The protein of approximately 49kDa was defined as human alpha-one antitrypsin (AT, from 23rd to 42nd amino acids of NH2-terminal) by amino acid sequence analysis. The ratio of the amount of AT to total proteins was 34.9% analyzed by densitometer. AT is presumably involved in stone formation as a coprecipitating substance because equilibrium dialysis revealed that AT has no affinity for calcium.