Protein tyrosine kinase 7 has a conserved role in Wnt/beta-catenin canonical signalling.
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Puppo F, Thome V, Lhoumeau AC, Cibois M, Gangar A, Lembo F, Belotti E, Marchetto S, Lecine P, Prebet T, Sebbagh M, Shin WS, Lee ST, Kodjabachian L, Borg JP
Protein tyrosine kinase 7 has a conserved role in Wnt/beta-catenin canonical signalling.
EMBO Rep. 2011 Jan;12(1):43-9. doi: 10.1038/embor.2010.185. Epub 2010 Dec 3.
- PubMed ID
- 21132015 [ View in PubMed]
- Abstract
The receptor protein tyrosine kinase 7 (PTK7) was recently shown to participate in noncanonical Wnt/planar cell polarity signalling during mouse and frog embryonic development. In this study, we report that PTK7 interacts with beta-catenin in a yeast two-hybrid assay and mammalian cells. PTK7-deficient cells exhibit weakened beta-catenin/T-cell factor transcriptional activity on Wnt3a stimulation. Furthermore, Xenopus PTK7 is required for the formation of Spemann's organizer and for Siamois promoter activation, events that require beta-catenin transcriptional activity. Using epistatic assays, we demonstrate that PTK7 functions upstream from glycogen synthase kinase 3. Taken together, our data reveal a new and conserved role for PTK7 in the Wnt canonical signalling pathway.