Retrovirus-mediated gene transfer of a human c-fos cDNA into mouse bone marrow stromal cells.
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Roux P, Verrier B, Klein B, Niccolino M, Marty L, Alexandre C, Piechaczyk M
Retrovirus-mediated gene transfer of a human c-fos cDNA into mouse bone marrow stromal cells.
Oncogene. 1991 Nov;6(11):2155-60.
- PubMed ID
- 1658710 [ View in PubMed]
- Abstract
A cDNA encoding a complete human c-fos protein was isolated and inserted into two different murine MoMuLV-derived recombinant retroviruses allowing expression of c-fos protein in different cell types. One c-fos-expressing retrovirus, chosen for its ability to express high levels of proteins in fibroblast-like cells, was shown to potentiate long-term cultures of mouse bone marrow stromal cells in vitro and therefore constitutes a potential tool for immortalizing such cells. Moreover, when tested in an in vitro differentiation assay, stromal cells constitutively expressing c-fos favor the granulocyte differentiation of hematopoietic precursors. Interestingly, retroviruses expressing v-src and v-abl oncogenes, included as controls in our experiments, do not produce any detectable effects, whereas those expressing polyoma virus middle T antigen facilitate long-term growth in vitro of stromal cells that favor the macrophage differentiation pathway of bone marrow stem cells. Our observation supports the idea that constitutive expression of some oncogenes, including c-fos and polyoma virus middle T antigen, may influence cytokine production by bone marrow stromal cells.