Organization and chromosomal localization of the human endothelial protein C receptor gene.
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Hayashi T, Nakamura H, Okada A, Takebayashi S, Wakita T, Yuasa H, Okumura K, Suzuki K
Organization and chromosomal localization of the human endothelial protein C receptor gene.
Gene. 1999 Oct 1;238(2):367-73.
- PubMed ID
- 10570964 [ View in PubMed]
- Abstract
Endothelial protein C receptor (EPCR), present on endothelial cells of relatively large veins and arteries, plays a role in the enhancement of protein C activation by the thrombin-thrombomodulin complex. In the present study, we determined the organization and the complete nucleotide sequence of the human EPCR gene using polymerase chain reaction-direct sequencing method. The transcription initiation site of the EPCR gene was also determined by the cap site hunting method, using a cap site cDNA prepared from human placenta. The human EPCR gene spanned approx. 6 kb and was composed of four exons and three introns. All exon-intron boundaries agreed with the GT-AG rule. The 5'-flanking region (300 bp) of the EPCR gene contained a putative AP1-binding site, two Sp1-binding sites and two AP2-binding sites, but not definite TATAA or CCAAT sequences. Fluorescence in situ hybridization analysis showed that the EPCR gene is located in chromosome 20q11.2.