2.0 A crystal structure of a four-domain segment of human fibronectin encompassing the RGD loop and synergy region.
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Leahy DJ, Aukhil I, Erickson HP
2.0 A crystal structure of a four-domain segment of human fibronectin encompassing the RGD loop and synergy region.
Cell. 1996 Jan 12;84(1):155-64.
- PubMed ID
- 8548820 [ View in PubMed]
- Abstract
We have determined the 2.0 A crystal structure of a fragment of human fibronectin encompassing the seventh through the RGD-containing tenth type III repeats (FN7-10). The structure reveals an extended rod-like molecule with a long axis of approximately 140 A and highly variable relationships between adjacent domains. An unusually small rotation between domains 9 and 10 creates a distinctive binding site, in which the RGD loop from domain 10 and the "synergy" region from domain 9 are on the same face of FN7-10 and thus easily accessible to a single integrin molecule. The cell-binding RGD loop is well-ordered in this structure and extends approximately 10 A away from the FN7-10 core.