Spectrofluorimetric determination of human serum albumin using terbium-danofloxacin probe.

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Ramezani AM, Manzoori JL, Amjadi M, Jouyban A

Spectrofluorimetric determination of human serum albumin using terbium-danofloxacin probe.

ScientificWorldJournal. 2012;2012:940541. doi: 10.1100/2012/940541. Epub 2012 May 2.

PubMed ID
22645474 [ View in PubMed
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Abstract

A spectrofluorimetric method is proposed for the determination of human serum albumin (HSA) and bovine serum albumin (BSA) using terbium-danofloxacin (Tb(3+)-Dano) as a fluorescent probe. These proteins remarkably enhance the fluorescence intensity of the Tb(3+)-Dano complex at 545 nm, and the enhanced fluorescence intensity of Tb(3+)-Dano is proportional to the concentration of proteins (HSA and BSA). Optimum conditions for the determination of HSA were investigated and found that the maximum response was observed at: pH = 7.8, [Tb(3+)] = 8.5 x 10(-5) mol L(-1), [Dano] = 1.5 x 10(-4) mol L(-1). The calibration graphs for standard solutions of BSA, HSA, and plasma samples of HSA were linear in the range of 0.2 x 10(-6) - 1.3 x 10(-6) mol L(-1), 0.2 x 10(-6) - 1.4 x 10(-6) mol L(-1), and 0.2 x 10(-6) - 1 x 10(-6) mol L(-1), respectively. The detection limits (S/N = 3) for BSA, HSA, and plasma sample of HSA were 8.7 x 10(-8) mol L(-1), 6.2 x 10(-8) mol L(-1), and 8.1 x 10(-8) mol L(-1), respectively. The applicability of the method was checked using a number of real biological plasma samples and was compared with the UV spectrometric reference method. The results was showed that the method could be regarded as a simple, practical, and sensitive alternative method for determination of albumin in biological samples.

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