Identification, isolation, and partial characterization of a 7.5-kDa surfactant-associated protein.
Article Details
- CitationCopy to clipboard
Singh G, Katyal SL, Brown WE, Kennedy AL, Wong-Chong ML, Gottron SA
Identification, isolation, and partial characterization of a 7.5-kDa surfactant-associated protein.
Exp Lung Res. 1991 May-Jun;17(3):559-67.
- PubMed ID
- 1860454 [ View in PubMed]
- Abstract
Human surfactant was analyzed for proteins associated with the lipids. Surfactant was isolated from lung lavage by the salt gradient centrifugation method, and the soluble proteins binding to the lipids were recovered by extraction with a low pH buffer. Antiserum to this preparation reacted with surfactant apoprotein A and a 7.5-kDa protein. The 7.5-kDa protein was isolated from reduced and alkylated lung lavage pellet by chromatography. The N-terminal amino acid sequence of the protein indicates that it is a novel protein.