Cbl promotes ubiquitination of the T cell receptor zeta through an adaptor function of Zap-70.
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Wang HY, Altman Y, Fang D, Elly C, Dai Y, Shao Y, Liu YC
Cbl promotes ubiquitination of the T cell receptor zeta through an adaptor function of Zap-70.
J Biol Chem. 2001 Jul 13;276(28):26004-11. Epub 2001 May 15.
- PubMed ID
- 11353765 [ View in PubMed]
- Abstract
Triggering of the T cell antigen receptor (TCR).CD3 complex induces its ubiquitination. However, the molecular events that lead to ubiquitin conjugation to these cell surface molecules have not been defined. Here we report that Cbl, a RING-type E3 ubiquitin-protein ligase, promotes ubiquitination of TCR zeta chain, which requires its functional variant Src homology 2 domain and an intact RING finger. The tyrosine kinase Zap-70, which binds to both TCR zeta and Cbl, plays an adaptor role in these events. Mutations in TCR zeta, Zap-70, or Cbl that disrupt the interaction between TCR zeta and Zap-70 or between Zap-70 and Cbl reduce ubiquitination of TCR zeta. Our results suggest a novel mechanism by which Cbl negatively regulates T cell development and activation by inducing ubiquitination of the TCR.CD3 components.