A glycyl radical site in the crystal structure of a class III ribonucleotide reductase.
Article Details
- CitationCopy to clipboard
Logan DT, Andersson J, Sjoberg BM, Nordlund P
A glycyl radical site in the crystal structure of a class III ribonucleotide reductase.
Science. 1999 Mar 5;283(5407):1499-504.
- PubMed ID
- 10066165 [ View in PubMed]
- Abstract
Ribonucleotide reductases catalyze the reduction of ribonucleotides to deoxyribonucleotides. Three classes have been identified, all using free-radical chemistry but based on different cofactors. Classes I and II have been shown to be evolutionarily related, whereas the origin of anaerobic class III has remained elusive. The structure of a class III enzyme suggests a common origin for the three classes but shows differences in the active site that can be understood on the basis of the radical-initiation system and source of reductive electrons, as well as a unique protein glycyl radical site. A possible evolutionary relationship between early deoxyribonucleotide metabolism and primary anaerobic metabolism is suggested.