Nucleotide sequencing and expression of the fadL gene involved in long-chain fatty acid transport in Escherichia coli.
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Said B, Ghosn CR, Vu L, Nunn WD
Nucleotide sequencing and expression of the fadL gene involved in long-chain fatty acid transport in Escherichia coli.
Mol Microbiol. 1988 May;2(3):363-70.
- PubMed ID
- 2840553 [ View in PubMed]
- Abstract
The fadL gene of Escherichia coli codes for an outer membrane protein involved in long-chain fatty acid transport. Its product was purified from outer membrane proteins. We determined the nucleotide sequence of a 2.8-kb chromosomal DNA segment that contains the fadL gene. The fadL gene consists of a 1149-nucleotide coding region and contains a highly hydrophobic polypeptide of 383 amino acids with a calculated molecular weight of 42,380. We have used S1-mapping analysis to identify the transcription initiation site. A region exhibiting extensive dyad symmetry and perfect homology to the catabolite activator protein binding site was detected.