Metabolism of L(-)-carnitine by Enterobacteriaceae under aerobic conditions.
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Elssner T, Preusser A, Wagner U, Kleber HP
Metabolism of L(-)-carnitine by Enterobacteriaceae under aerobic conditions.
FEMS Microbiol Lett. 1999 May 15;174(2):295-301.
- PubMed ID
- 10339822 [ View in PubMed]
- Abstract
Different Enterobacteriaceae, such as Escherichia coli, Proteus vulgaris and Proteus mirabilis, are able to convert L(-)-carnitine, via crotonobetaine, into gamma-butyrobetaine in the presence of carbon and nitrogen sources under aerobic conditions. Intermediates of L(-)-carnitine metabolism (crotonobetaine, gamma-butyrobetaine) could be detected by thin-layer chromatography. In parallel, L(-)-carnitine dehydratase, carnitine racemasing system and crotonobetaine reductase activities were determined enzymatically. Monoclonal antibodies against purified CaiB and CaiA from E. coli O44K74 were used to screen cell-free extracts of different Enterobacteriaceae (E. coli ATCC 25922, P. vulgaris, P. mirabilis, Citrobacter freundii, Enterobacter cloacae and Klebsiella pneumoniae) grown under aerobic conditions in the presence of L(-)-carnitine.