Cloning and characterisation of the gene encoding cytochrome c4 from Pseudomonas stutzeri.
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Christensen HE
Cloning and characterisation of the gene encoding cytochrome c4 from Pseudomonas stutzeri.
Gene. 1994 Jun 24;144(1):139-40.
- PubMed ID
- 8026750 [ View in PubMed]
- Abstract
By use of a degenerate oligodeoxyribonucleotide probe corresponding to the N-terminal amino acid (aa) sequence and Southern blot analysis, the gene encoding the di-heme cytochrome c4 (CC4) from Pseudomonas stutzeri has been cloned. The aa sequence deduced from the nucleotide sequence shows a 20-aa signal peptide and a 190-aa mature protein with 79% identity to the Azotobacter vinelandii CC4 sequence, which had earlier been determined by aa sequencing.