Regulatory pattern of the Escherichia coli lysA gene: expression of chromosomal lysA-lacZ fusions.
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Stragier P, Borne F, Richaud F, Richaud C, Patte JC
Regulatory pattern of the Escherichia coli lysA gene: expression of chromosomal lysA-lacZ fusions.
J Bacteriol. 1983 Dec;156(3):1198-203.
- PubMed ID
- 6417111 [ View in PubMed]
- Abstract
The regulation of lysA which encodes the last enzyme for lysine biosynthesis in Escherichia coli, diaminopimelic acid-decarboxylase, was studied by using lysA-lacZ fusions. Our results indicate an absolute requirement for the LysR product for its activation, LysR protein present in a limiting amount which can be titrated by a multicopy plasmid carrying its target site and a negative regulatory role for the LysA protein itself which decreases lysA-lacZ expression 30-fold.