The apoptosis-associated protein BNIPL interacts with two cell proliferation-related proteins, MIF and GFER.
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Shen L, Hu J, Lu H, Wu M, Qin W, Wan D, Li YY, Gu J
The apoptosis-associated protein BNIPL interacts with two cell proliferation-related proteins, MIF and GFER.
FEBS Lett. 2003 Apr 10;540(1-3):86-90.
- PubMed ID
- 12681488 [ View in PubMed]
- Abstract
Bcl-2/adenovirus E1B 19 kDa interacting protein 2-like, BNIP-2-like (BNIPL) is a recently cloned and characterized apoptosis-associated protein that shares 72% homology with BNIP-2. It is highly expressed in human placenta and lung. A yeast two-hybrid system was used to obtain two BNIPL-interacting proteins, MIF (macrophage migration inhibitory factor) and GFER (growth factor erv1 (Saccharomyces cerevisiae)-like). The interactions were confirmed by glutathione S-transferase pull-down assay in vitro and co-immunoprecipitation assay in vivo. Colony formation assay and cell proliferation test suggest that overexpression of BNIPL could inhibit the growth of BEL-7402 cells. These findings suggest that BNIPL may physically bind to cell proliferation-related proteins, MIF and GFER.