The structural basis of specific base-excision repair by uracil-DNA glycosylase.
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Savva R, McAuley-Hecht K, Brown T, Pearl L
The structural basis of specific base-excision repair by uracil-DNA glycosylase.
Nature. 1995 Feb 9;373(6514):487-93.
- PubMed ID
- 7845459 [ View in PubMed]
- Abstract
The 1.75-A crystal structure of the uracil-DNA glycosylase from herpes simplex virus type-1 reveals a new fold, distantly related to dinucleotide-binding proteins. Complexes with a trideoxynucleotide, and with uracil, define the DNA-binding site and allow a detailed understanding of the exquisitely specific recognition of uracil in DNA. The overall structure suggests binding models for elongated single- and double-stranded DNA substrates. Conserved residues close to the uracil-binding site suggest a catalytic mechanism for hydrolytic base excision.