Protein EnvM is the NADH-dependent enoyl-ACP reductase (FabI) of Escherichia coli.
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Bergler H, Wallner P, Ebeling A, Leitinger B, Fuchsbichler S, Aschauer H, Kollenz G, Hogenauer G, Turnowsky F
Protein EnvM is the NADH-dependent enoyl-ACP reductase (FabI) of Escherichia coli.
J Biol Chem. 1994 Feb 25;269(8):5493-6.
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- 8119879 [ View in PubMed]
- Abstract
The EnvM protein was purified from an overproducing Escherichia coli strain. It showed NADH-dependent enoyl-acyl carrier protein (ACP) reductase activity using both crotonyl-ACP and crotonyl-CoA as substrates. The protein bound a radioactive diazaborine derivative in the presence of NAD+ and radioactive NAD+ in the presence of the drug. Based on these data, it is concluded that EnvM is the NADH-dependent enoyl-ACP reductase (EC 1.3.1.9) of E. coli and we propose to rename the corresponding gene fabI.