Sequencing of cDNA inserts encoding aromatase cytochrome P-450 (P-450AROM).
Article Details
- CitationCopy to clipboard
Simpson ER, Evans CT, Corbin CJ, Powell FE, Ledesma DB, Mendelson CR
Sequencing of cDNA inserts encoding aromatase cytochrome P-450 (P-450AROM).
Mol Cell Endocrinol. 1987 Aug;52(3):267-72.
- PubMed ID
- 3653507 [ View in PubMed]
- Abstract
Two cDNA inserts complementary to mRNA encoding aromatase cytochrome P-450 (P-450AROM) have been isolated and characterized by restriction mapping and sequencing. The overlapping sequence encoded by these inserts is identical, and a putative heme-binding region has been identified. In addition, the open reading frame contains the sequences of all four cysteine-containing tryptic peptides isolated by Chen et al. (1986) from purified cytochrome P-450AROM. The inserts differ in the use of alternative poly A-addition signals, which is consistent with the presence of two major species of mRNA in human placenta, of 3.0 and 2.4 kb, which hybridize to these inserts. The identity of sequence between the two inserts and the likely presence of alternative poly A-addition signals, is suggestive that only one form of cytochrome P-450AROM is encoded by these mRNA species.