Expression of recombinant platelet-derived endothelial cell growth factor in the yeast Saccharomyces cerevisiae.
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Finnis C, Goodey A, Courtney M, Sleep D
Expression of recombinant platelet-derived endothelial cell growth factor in the yeast Saccharomyces cerevisiae.
Yeast. 1992 Jan;8(1):57-60.
- PubMed ID
- 1580101 [ View in PubMed]
- Abstract
A platelet-derived endothelial cell growth factor cDNA has been cloned, sequenced and expressed using the Saccharomyces cerevisiae PRB1 promoter. Soluble recombinant platelet-derived endothelial cell growth factor constituted 0.5-1.0% of total soluble protein. Yeast soluble protein extracts containing recombinant platelet-derived endothelial cell growth factor stimulate the growth of calf pulmonary artery endothelial cells in vitro.