Structures of native human thymidine phosphorylase and in complex with 5-iodouracil.
Article Details
- CitationCopy to clipboard
Mitsiki E, Papageorgiou AC, Iyer S, Thiyagarajan N, Prior SH, Sleep D, Finnis C, Acharya KR
Structures of native human thymidine phosphorylase and in complex with 5-iodouracil.
Biochem Biophys Res Commun. 2009 Sep 4;386(4):666-70. doi: 10.1016/j.bbrc.2009.06.104. Epub 2009 Jun 23.
- PubMed ID
- 19555658 [ View in PubMed]
- Abstract
Thymidine phosphorylase (TP) first identified as platelet derived endothelial cell growth factor (PD-ECGF) plays a key role in nucleoside metabolism. Human TP (hTP) is implicated in angiogenesis and is overexpressed in several solid tumors. Here, we report the crystal structures of recombinant hTP and its complex with a substrate 5-iodouracil (5IUR) at 3.0 and 2.5A, respectively. In addition, we provide information on the role of specific residues in the enzymatic activity of hTP through mutagenesis and kinetic studies.