Aklanonic acid methyl ester cyclase AcmA
Details
- Name
- Aklanonic acid methyl ester cyclase AcmA
- Kind
- protein
- Synonyms
- 5.5.1.23
- AAME cyclase
- aknH
- Methyl aklanonate cyclase
- Gene Name
- acma
- UniProtKB Entry
- O52646Swiss-Prot
- Organism
- Streptomyces galilaeus
- NCBI Taxonomy ID
- 33899
- Amino acid sequence
>lcl|BSEQ0022075|Aklanonic acid methyl ester cyclase AcmA MSEQIAAVRRMVEAYNTGKTDDVADYIHPEYMNPGTLEFTSLRGPELFAINVAWVKKTFS EEARLEEVGIEERADWVRARLVLYGRHVGEMVGMAPTGRLFSGEQIHLLHFVDGKIHHHR DWPDYQGTYRQLGEPWPETEHRRP
- Number of residues
- 144
- Molecular Weight
- 16730.765
- Theoretical pI
- 6.08
- GO Classification
- Functionsintramolecular lyase activityProcessesantibiotic biosynthetic process / daunorubicin biosynthetic process / doxorubicin metabolic process
- General Function
- Involved in the biosynthesis of aklavinone which is an important precursor common to the formation of the clinically significant anthracyclines such as carminomycin, daunorubicin (daunomycin), rhodomycin, aclacinomycin T (aklavin) and aclacinomycin A (aclarubicin). These compounds are aromatic polyketide antibiotics that exhibit high cytotoxicity and are widely applied in the chemotherapy of a variety of cancers. Catalyzes the cyclization of aklanonic acid methyl ester to yield aklaviketone. It is also able to use nogalonic acid methyl ester as substrate, but produces exclusively auraviketone with C9-R stereochemistry.
- Specific Function
- intramolecular lyase activity
- Pfam Domain Function
- SnoaL (PF07366)
- Signal Regions
- Not Available
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0006516|435 bp GTGAGTGAGCAGATAGCGGCCGTTCGGCGCATGGTCGAGGCGTACAACACCGGAAAAACG GATGATGTGGCCGACTACATCCATCCGGAATACATGAATCCGGGAACCCTGGAATTCACC TCGCTGCGTGGCCCGGAACTCTTCGCGATCAATGTCGCGTGGGTCAAGAAGACGTTCTCC GAGGAGGCGCGGCTGGAAGAGGTCGGCATCGAGGAGCGGGCCGACTGGGTGCGCGCCCGG CTGGTGCTCTACGGCCGGCACGTCGGTGAGATGGTCGGCATGGCGCCGACCGGACGGCTG TTCTCCGGCGAGCAGATCCATCTCCTGCACTTCGTCGACGGGAAGATCCACCACCACCGC GACTGGCCCGACTACCAGGGCACCTACCGCCAGCTCGGCGAGCCGTGGCCGGAGACCGAG CACCGCCGTCCCTGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID O52646 UniProtKB Entry Name DNRD_STRGJ GenBank Gene ID AF043550 PDB ID(s) 2F98, 2F99 KEGG ID ag:AAF70112 - General References
- Kantola J, Kunnari T, Hautala A, Hakala J, Ylihonko K, Mantsala P: Elucidation of anthracyclinone biosynthesis by stepwise cloning of genes for anthracyclines from three different Streptomyces spp. Microbiology. 2000 Jan;146 ( Pt 1):155-63. [Article]
- Raty K, Kantola J, Hautala A, Hakala J, Ylihonko K, Mantsala P: Cloning and characterization of Streptomyces galilaeus aclacinomycins polyketide synthase (PKS) cluster. Gene. 2002 Jun 26;293(1-2):115-22. [Article]
- Chung JY, Fujii I, Harada S, Sankawa U, Ebizuka Y: Expression, purification, and characterization of AknX anthrone oxygenase, which is involved in aklavinone biosynthesis in Streptomyces galilaeus. J Bacteriol. 2002 Nov;184(22):6115-22. [Article]
- Kallio P, Sultana A, Niemi J, Mantsala P, Schneider G: Crystal structure of the polyketide cyclase AknH with bound substrate and product analogue: implications for catalytic mechanism and product stereoselectivity. J Mol Biol. 2006 Mar 17;357(1):210-20. Epub 2006 Jan 6. [Article]
Associated Data
- Drug Relations
Drug Drug group Pharmacological action? Type Actions Details DERIVATIVE OF AKLANONIC ACID METHYL ESTER (AAME) experimental unknown target Details