Peptide deformylase

Details

Name
Peptide deformylase
Kind
protein
Synonyms
  • 3.5.1.88
  • pdf
  • Polypeptide deformylase
Gene Name
def
UniProtKB Entry
Q93LE9Swiss-Prot
Organism
Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
NCBI Taxonomy ID
189518
Amino acid sequence
>lcl|BSEQ0019482|Peptide deformylase
MSVRKILRMGDPILRKISEPVTEDEIQTKEFKKLIRDMFDTMRHAEGVGLAAPQIGILKQ
IVVVGSEDNERYPGTPDVPERIILNPVITPLTKDTSGFWEGCLSVPGMRGYVERPNQIRM
QWMDEKGNQFDETIDGYKAIVYQHECDHLQGILYVDRLKDTKLFGFNETLDSSHNVLD
Number of residues
178
Molecular Weight
20379.185
Theoretical pI
4.93
GO Classification
Functions
iron ion binding / peptide deformylase activity
Processes
translation
General Function
Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions (By similarity).
Specific Function
metal ion binding
Pfam Domain Function
Signal Regions
Not Available
Transmembrane Regions
Not Available
Cellular Location
Cytoplasmic
Gene sequence
>lcl|BSEQ0019483|Peptide deformylase (def)
ATGTCAGTCAGAAAAATTTTAAGAATGGGAGATCCAATTCTCCGTAAAATCTCAGAGCCA
GTTACAGAAGACGAAATTCAAACTAAAGAATTCAAAAAGTTAATCCGAGATATGTTCGAT
ACAATGCGACACGCGGAAGGAGTGGGACTTGCGGCTCCTCAGATCGGAATTTTGAAACAA
ATCGTAGTCGTTGGTTCCGAAGATAATGAACGTTACCCAGGAACCCCAGACGTTCCAGAG
AGAATCATTTTAAATCCAGTAATTACACCTCTTACAAAAGATACTTCCGGTTTTTGGGAA
GGTTGCCTTTCCGTCCCAGGAATGCGCGGTTATGTGGAAAGACCCAACCAAATCAGAATG
CAATGGATGGACGAAAAAGGAAACCAATTTGACGAAACCATAGACGGCTATAAAGCAATC
GTCTATCAACATGAATGTGATCACCTACAGGGAATTTTATACGTAGATAGACTAAAAGAC
ACTAAGTTATTCGGTTTTAACGAAACCCTAGACTCTTCTCACAACGTCCTAGACTAA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDQ93LE9
UniProtKB Entry NameDEF_LEPIN
GenBank Protein ID14626937
GenBank Gene IDAY040678
PDB ID(s)1SV2, 1SZZ, 1VEV, 1VEY, 1VEZ, 1Y6H
KEGG IDlil:LA_2438
NCBI Gene ID1151781
General References
  1. Li Y, Ren S, Gong W: Cloning, high-level expression, purification and crystallization of peptide deformylase from Leptospira interrogans. Acta Crystallogr D Biol Crystallogr. 2002 May;58(Pt 5):846-8. Epub 2002 Apr 26. [Article]
  2. Ren SX, Fu G, Jiang XG, Zeng R, Miao YG, Xu H, Zhang YX, Xiong H, Lu G, Lu LF, Jiang HQ, Jia J, Tu YF, Jiang JX, Gu WY, Zhang YQ, Cai Z, Sheng HH, Yin HF, Zhang Y, Zhu GF, Wan M, Huang HL, Qian Z, Wang SY, Ma W, Yao ZJ, Shen Y, Qiang BQ, Xia QC, Guo XK, Danchin A, Saint Girons I, Somerville RL, Wen YM, Shi MH, Chen Z, Xu JG, Zhao GP: Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing. Nature. 2003 Apr 24;422(6934):888-93. [Article]
  3. Zhou Z, Song X, Li Y, Gong W: Unique structural characteristics of peptide deformylase from pathogenic bacterium Leptospira interrogans. J Mol Biol. 2004 May 21;339(1):207-15. [Article]

Associated Data

Drug Relations
DrugDrug groupPharmacological action?TypeActionsDetails
2-[(Formyl-Hydroxy-Amino)-Methyl]-Heptanoic Acid [1-(2-Hydroxymethyl-Pyrrolidine-1-Carbonyl)-2-Methyl-Propyl]-AmideexperimentalunknowntargetDetails