Type-2 restriction enzyme EcoRV
Details
- Name
- Type-2 restriction enzyme EcoRV
- Kind
- protein
- Synonyms
- 3.1.21.4
- Endonuclease EcoRV
- R.EcoRV
- Type II restriction enzyme EcoRV
- Gene Name
- ecoRVR
- UniProtKB Entry
- P04390Swiss-Prot
- Organism
- Escherichia coli
- NCBI Taxonomy ID
- 562
- Amino acid sequence
>lcl|BSEQ0017421|Type-2 restriction enzyme EcoRV MSLRSDLINALYDENQKYDVCGIISAEGKIYPLGSDTKVLSTIFELFSRPIINKIAEKHG YIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIKTTYTNKENEKIKFTLGGYTSFIRNNTKN IVYPFDQYIAHWIIGYVYTRVATRKSSLKTYNINELNEIPKPYKGVKVFLQDKWVIAGDL AGSGNTTNIGSIHAHYKDFVEGKGIFDSEDEFLDYWRNYERTSQLRNDKYNNISEYRNWI YRGRK
- Number of residues
- 245
- Molecular Weight
- 28650.14
- Theoretical pI
- 9.28
- GO Classification
- FunctionsDNA binding / metal ion binding / Type II site-specific deoxyribonuclease activityProcessesDNA restriction-modification system
- General Function
- A P subtype restriction enzyme that recognizes the double-stranded sequence 5'-GATATC-3' and cleaves after T-3.
- Specific Function
- DNA binding
- Pfam Domain Function
- Endonuc-EcoRV (PF09233)
- Signal Regions
- Not Available
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0017422|Type-2 restriction enzyme EcoRV (ecoRVR) ATGAGTCTTCGTTCTGATTTAATTAATGCACTATATGATGAAAATCAAAAATATGATGTA TGCGGAATAATATCTGCAGAAGGAAAAATATACCCATTGGGAAGTGACACAAAAGTTCTA AGCACAATATTTGAGTTATTCTCAAGACCAATAATAAATAAAATAGCAGAAAAACATGGG TATATTGTAGAAGAACCTAAACAACAAAATCATTATCCTGACTTTACTCTTTACAAACCA AGCGAACCAAATAAAAAAATTGCAATAGATATAAAAACAACATATACAAACAAAGAAAAC GAAAAAATCAAGTTCACTCTTGGTGGGTATACCAGCTTTATACGAAACAACACAAAAAAT ATTGTTTATCCATTTGACCAATATATCGCCCATTGGATAATCGGATATGTATATACAAGA GTTGCTACAAGAAAATCATCTTTAAAAACATATAATATAAATGAACTCAATGAAATCCCT AAACCATACAAAGGCGTAAAGGTTTTCTTACAAGATAAATGGGTTATTGCTGGAGATTTG GCAGGATCTGGAAACACAACAAATATAGGTAGCATTCATGCCCACTATAAAGACTTTGTA GAAGGAAAAGGAATATTTGACTCAGAGGATGAGTTTTTAGACTATTGGAGAAATTATGAA AGAACCAGTCAATTAAGAAATGACAAGTATAATAATATAAGCGAATACAGAAACTGGATA TACCGAGGAAGAAAATAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P04390 UniProtKB Entry Name T2E5_ECOLX GenBank Protein ID 41325 GenBank Gene ID X00530 PDB ID(s) 1AZ0, 1AZ3, 1AZ4, 1B94, 1B95, 1B96, 1B97, 1BGB, 1BSS, 1BSU, 1BUA, 1EO3, 1EO4, 1EON, 1EOO, 1EOP, 1RV5, 1RVA, 1RVB, 1RVC, 1RVE, 1STX, 1SUZ, 1SX5, 1SX8, 2B0D, 2B0E, 2GE5, 2RVE, 4ORJ, 4RVE KEGG ID pg:14401263 NCBI Gene ID 14401263 - General References
- Bougueleret L, Schwarzstein M, Tsugita A, Zabeau M: Characterization of the genes coding for the Eco RV restriction and modification system of Escherichia coli. Nucleic Acids Res. 1984 Apr 25;12(8):3659-76. [Article]
- Thielking V, Selent U, Kohler E, Wolfes H, Pieper U, Geiger R, Urbanke C, Winkler FK, Pingoud A: Site-directed mutagenesis studies with EcoRV restriction endonuclease to identify regions involved in recognition and catalysis. Biochemistry. 1991 Jul 2;30(26):6416-22. [Article]
- Winkler FK, Banner DW, Oefner C, Tsernoglou D, Brown RS, Heathman SP, Bryan RK, Martin PD, Petratos K, Wilson KS: The crystal structure of EcoRV endonuclease and of its complexes with cognate and non-cognate DNA fragments. EMBO J. 1993 May;12(5):1781-95. [Article]
- Kostrewa D, Winkler FK: Mg2+ binding to the active site of EcoRV endonuclease: a crystallographic study of complexes with substrate and product DNA at 2 A resolution. Biochemistry. 1995 Jan 17;34(2):683-96. [Article]
- Perona JJ, Martin AM: Conformational transitions and structural deformability of EcoRV endonuclease revealed by crystallographic analysis. J Mol Biol. 1997 Oct 17;273(1):207-25. [Article]
- Horton NC, Perona JJ: Recognition of flanking DNA sequences by EcoRV endonuclease involves alternative patterns of water-mediated contacts. J Biol Chem. 1998 Aug 21;273(34):21721-9. [Article]
- Horton NC, Perona JJ: Role of protein-induced bending in the specificity of DNA recognition: crystal structure of EcoRV endonuclease complexed with d(AAAGAT) + d(ATCTT). J Mol Biol. 1998 Apr 10;277(4):779-87. [Article]
- Thomas MP, Brady RL, Halford SE, Sessions RB, Baldwin GS: Structural analysis of a mutational hot-spot in the EcoRV restriction endonuclease: a catalytic role for a main chain carbonyl group. Nucleic Acids Res. 1999 Sep 1;27(17):3438-45. [Article]
Associated Data
- Drug Relations
Drug Drug group Pharmacological action? Type Actions Details 3'-THIO-THYMIDINE-5'-PHOSPHATE experimental unknown target Details