Guanine nucleotide-binding protein G(i) subunit alpha-1
Details
- Name
- Guanine nucleotide-binding protein G(i) subunit alpha-1
- Kind
- protein
- Synonyms
- 3.6.5.-
- Adenylate cyclase-inhibiting G alpha protein
- Gene Name
- GNAI1
- UniProtKB Entry
- P63096Swiss-Prot
- Organism
- Humans
- NCBI Taxonomy ID
- 9606
- Amino acid sequence
>lcl|BSEQ0009412|Guanine nucleotide-binding protein G(i) subunit alpha-1 MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAG YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK TTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM NRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA AYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF
- Number of residues
- 354
- Molecular Weight
- 40360.685
- Theoretical pI
- Not Available
- GO Classification
- FunctionsD2 dopamine receptor binding / G protein-coupled receptor binding / G protein-coupled serotonin receptor binding / magnesium ion bindingProcessesadenylate cyclase-inhibiting G protein-coupled receptor signaling pathway / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / cellular response to forskolin / G protein-coupled receptor signaling pathway / positive regulation of protein localization to cell cortex / regulation of cAMP-mediated signaling / regulation of mitotic spindle organization / T cell migrationComponentscell cortex / nucleolus / nucleoplasm
- General Function
- Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed:18434541, PubMed:8774883, PubMed:34239069, PubMed:33762731, PubMed:35610220, PubMed:37963465, PubMed:37935377, PubMed:37935376, PubMed:38552625). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed:18434541, PubMed:8774883). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed:18434541, PubMed:8774883). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed:18434541, PubMed:8774883). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:18434541, PubMed:8774883). Signaling is mediated via effector proteins, such as adenylate cyclase: inhibits adenylate cyclase activity of ADCY1, ADCY5 and ADCY6, leading to decreased intracellular cAMP levels (PubMed:8119955). The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. Required for normal cytokinesis during mitosis (PubMed:17635935). Required for cortical dynein-dynactin complex recruitment during metaphase (PubMed:22327364)
- Specific Function
- D2 dopamine receptor binding
- Pfam Domain Function
- G-alpha (PF00503)
- Signal Regions
- Not Available
- Transmembrane Regions
- Not Available
- Cellular Location
- Nucleus
- Gene sequence
>lcl|BSEQ0017452|Guanine nucleotide-binding protein G(i) subunit alpha-1 (GNAI1) ATGAAAATTATCCATGAAGCTGGTTATTCAGAAGAGGAGTGTAAACAATACAAAGCAGTG GTCTACAGTAACACCATCCAGTCAATTATTGCTATCATTAGGGCTATGGGGAGGTTGAAG ATAGACTTTGGTGACTCAGCCCGGGCGGATGATGCACGCCAACTCTTTGTGCTAGCTGGA GCTGCTGAAGAAGGCTTTATGACTGCAGAACTTGCTGGAGTTATAAAGAGATTGTGGAAA GATAGTGGTGTACAAGCCTGTTTCAACAGATCCCGAGAGTACCAGCTTAATGATTCTGCA GCATACTATTTGAATGACTTGGACAGAATAGCTCAACCAAATTACATCCCGACTCAACAA GATGTTCTCAGAACTAGAGTGAAAACTACAGGAATTGTTGAAACCCATTTTACTTTCAAA GATCTTCATTTTAAAATGTTTGATGTGGGAGGTCAGAGATCTGAGCGGAAGAAGTGGATT CATTGCTTCGAAGGAGTGACGGCGATCATCTTCTGTGTAGCACTGAGTGACTACGACCTG GTTCTAGCTGAAGATGAAGAAATGAACCGAATGCATGAAAGCATGAAATTGTTTGACAGC ATATGTAACAACAAGTGGTTTACAGATACATCCATTATACTTTTTCTAAACAAGAAGGAT CTCTTTGAAGAAAAAATCAAAAAGAGCCCTCTCACTATATGCTATCCAGAATATGCAGGA TCAAACACATATGAAGAGGCAGCTGCATATATTCAATGTCAGTTTGAAGACCTCAATAAA AGAAAGGACACAAAGGAAATATACACCCACTTCACATGTGCCACAGATACTAAGAATGTG CAGTTTGTTTTTGATGCTGTAACAGATGTCATCATAAAAAATAATCTAAAAGATTGTGGT CTCTTTTAA
- Chromosome Location
- 7
- Locus
- 7q21.11
- External Identifiers
- General References
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Associated Data
- Drug Relations
Drug Drug group Pharmacological action? Type Actions Details Guanosine-5'-Diphosphate experimental unknown target Details Tetrafluoroaluminate Ion experimental unknown target Details