Guanine nucleotide-binding protein G(i) subunit alpha-1

Details

Name
Guanine nucleotide-binding protein G(i) subunit alpha-1
Kind
protein
Synonyms
  • 3.6.5.-
  • Adenylate cyclase-inhibiting G alpha protein
Gene Name
GNAI1
UniProtKB Entry
P63096Swiss-Prot
Organism
Humans
NCBI Taxonomy ID
9606
Amino acid sequence
>lcl|BSEQ0009412|Guanine nucleotide-binding protein G(i) subunit alpha-1
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF
Number of residues
354
Molecular Weight
40360.685
Theoretical pI
Not Available
GO Classification
Functions
D2 dopamine receptor binding / G protein-coupled receptor binding / G protein-coupled serotonin receptor binding / magnesium ion binding
Processes
adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / cellular response to forskolin / G protein-coupled receptor signaling pathway / positive regulation of protein localization to cell cortex / regulation of cAMP-mediated signaling / regulation of mitotic spindle organization / T cell migration
Components
cell cortex / nucleolus / nucleoplasm
General Function
Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed:18434541, PubMed:8774883, PubMed:34239069, PubMed:33762731, PubMed:35610220, PubMed:37963465, PubMed:37935377, PubMed:37935376, PubMed:38552625). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed:18434541, PubMed:8774883). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed:18434541, PubMed:8774883). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed:18434541, PubMed:8774883). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:18434541, PubMed:8774883). Signaling is mediated via effector proteins, such as adenylate cyclase: inhibits adenylate cyclase activity of ADCY1, ADCY5 and ADCY6, leading to decreased intracellular cAMP levels (PubMed:8119955). The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. Required for normal cytokinesis during mitosis (PubMed:17635935). Required for cortical dynein-dynactin complex recruitment during metaphase (PubMed:22327364)
Specific Function
D2 dopamine receptor binding
Pfam Domain Function
Signal Regions
Not Available
Transmembrane Regions
Not Available
Cellular Location
Nucleus
Gene sequence
>lcl|BSEQ0017452|Guanine nucleotide-binding protein G(i) subunit alpha-1 (GNAI1)
ATGAAAATTATCCATGAAGCTGGTTATTCAGAAGAGGAGTGTAAACAATACAAAGCAGTG
GTCTACAGTAACACCATCCAGTCAATTATTGCTATCATTAGGGCTATGGGGAGGTTGAAG
ATAGACTTTGGTGACTCAGCCCGGGCGGATGATGCACGCCAACTCTTTGTGCTAGCTGGA
GCTGCTGAAGAAGGCTTTATGACTGCAGAACTTGCTGGAGTTATAAAGAGATTGTGGAAA
GATAGTGGTGTACAAGCCTGTTTCAACAGATCCCGAGAGTACCAGCTTAATGATTCTGCA
GCATACTATTTGAATGACTTGGACAGAATAGCTCAACCAAATTACATCCCGACTCAACAA
GATGTTCTCAGAACTAGAGTGAAAACTACAGGAATTGTTGAAACCCATTTTACTTTCAAA
GATCTTCATTTTAAAATGTTTGATGTGGGAGGTCAGAGATCTGAGCGGAAGAAGTGGATT
CATTGCTTCGAAGGAGTGACGGCGATCATCTTCTGTGTAGCACTGAGTGACTACGACCTG
GTTCTAGCTGAAGATGAAGAAATGAACCGAATGCATGAAAGCATGAAATTGTTTGACAGC
ATATGTAACAACAAGTGGTTTACAGATACATCCATTATACTTTTTCTAAACAAGAAGGAT
CTCTTTGAAGAAAAAATCAAAAAGAGCCCTCTCACTATATGCTATCCAGAATATGCAGGA
TCAAACACATATGAAGAGGCAGCTGCATATATTCAATGTCAGTTTGAAGACCTCAATAAA
AGAAAGGACACAAAGGAAATATACACCCACTTCACATGTGCCACAGATACTAAGAATGTG
CAGTTTGTTTTTGATGCTGTAACAGATGTCATCATAAAAAATAATCTAAAAGATTGTGGT
CTCTTTTAA
Chromosome Location
7
Locus
7q21.11
External Identifiers
ResourceLink
UniProtKB IDP63096
UniProtKB Entry NameGNAI1_HUMAN
GeneCard IDGNAI1
HGNC IDHGNC:4384
PDB ID(s)1KJY, 1Y3A, 2G83, 2GTP, 2IK8, 2OM2, 2XNS, 3ONW, 3QE0, 3QI2, 3UMR, 3UMS, 4G5Q, 5JS7, 5JS8, 5TDH, 6CMO, 6CRK, 6DDE, 6DDF, 6KPF, 6KPG, 6LFM, 6LFO, 6LML, 6N4B, 6OMM, 6OS9, 6OSA, 6OT0, 6PB0, 6PB1, 6PT0, 6QNO, 6VU8, 6XBJ, 6XBK, 6XBL, 6XBM, 7CMU, 7CMV, 7DB6, 7DW9, 7E2X, 7E2Y, 7E2Z, 7E32, 7E33, 7E9G, 7EB2, 7EJX, 7EO2, 7EO4, 7EUO, 7EVY, 7EVZ, 7EW0, 7EW1, 7EW2, 7EW3, 7EW4, 7EW7, 7EXD, 7EZH, 7EZK, 7EZM, 7F1Q, 7F1R, 7F1S, 7F4D, 7F4F, 7F4H, 7F4I, 7JHJ, 7JVR, 7L0P, 7L0Q, 7L0R, 7L0S, 7MTS, 7NA7, 7NA8, 7O7F, 7P02, 7RKM, 7RKN, 7RKX, 7RKY, 7S8M, 7S8O, 7SBF, 7SCG, 7SQO, 7T2G, 7T2H, 7T6B, 7T6S, 7T6T, 7T6U, 7T6V, 7TRK, 7TRP, 7TRQ, 7TRS, 7TUZ, 7U2K, 7U2L, 7V68, 7V69, 7V6A, 7V9L, 7VAB, 7VDH, 7VDL, 7VDM, 7VFX, 7VGX, 7VGY, 7VGZ, 7VH0, 7VIE, 7VIF, 7VIG, 7VIH, 7VKT, 7VL8, 7VL9, 7VLA, 7VUG, 7VUY, 7VUZ, 7VV3, 7VV5, 7W0L, 7W0M, 7W0N, 7W0O, 7W0P, 7WF7, 7WIC, 7WIG, 7WJ5, 7WQ3, 7WU4, 7WU5, 7WU9, 7WUI, 7WUJ, 7WVU, 7WYB, 7WZ4, 7X2V, 7X5H, 7X9A, 7X9B, 7X9C, 7X9Y, 7XA3, 7XAT, 7XAU, 7XAV, 7XBW, 7XBX, 7XK2, 7XK8, 7XMR, 7XMS, 7XMT, 7XTA, 7XXH, 7Y12, 7Y15, 7Y1F, 7Y64, 7Y65, 7Y66, 7Y67, 7Y89, 7YAC, 7YAE, 7YDJ, 7YDP, 7YKD, 7YON, 7YOO, 7YU3, 7YU5, 7YU6, 7YU7, 7YU8, 8DZP, 8EF5, 8EF6, 8EFB, 8EFL, 8EFO, 8EFQ, 8F7Q, 8F7R, 8F7S, 8F7W, 8F7X, 8FEG, 8FY8, 8FYE, 8FYL, 8FYT, 8FYX, 8G05, 8G59, 8G94, 8GAG, 8GCM, 8GCP, 8GDC, 8GHV, 8GUQ, 8GUR, 8GUS, 8GUT, 8H2G, 8H4I, 8HBD, 8HK2, 8HK3, 8HK5, 8HN8, 8HNK, 8HNL, 8HNM, 8HOC, 8HQE, 8HQM, 8HQN, 8HS3, 8HSC, 8HVI, 8I7V, 8I7W, 8IA8, 8IC0, 8ID3, 8ID4, 8ID6, 8ID8, 8ID9, 8IHB, 8IHF, 8IHH, 8IHI, 8IHJ, 8IJ3, 8IJA, 8IJB, 8IJD, 8IKG, 8IKH, 8INR, 8IOC, 8IOD, 8IRS, 8IRT, 8IRV, 8IU2, 8IW4, 8IW7, 8IW9, 8IY5, 8IZ4, 8J18, 8J19, 8J1A, 8J20, 8J21, 8J22, 8J23, 8J24, 8J6I, 8J6J, 8J6L, 8J6P, 8J6Q, 8J6R, 8JD3, 8JD5, 8JGB, 8JGG, 8JHY, 8JII, 8JIL, 8JIM, 8JIP, 8JIR, 8JIS, 8JLJ, 8JLK, 8JLR, 8JPN, 8JR9, 8JSO, 8JSP, 8JT6, 8JXT, 8JXV, 8JXW, 8JXX, 8JZ7, 8K2X, 8K4N, 8KFX, 8KFY, 8KFZ, 8PJK, 8PKM, 8SAI, 8SG1, 8U1U, 8U4N, 8U4O, 8U4P, 8U4Q, 8UGY, 8UH3, 8VY7, 8W8B, 8W8R, 8WC3, 8WC4, 8WC5, 8WC6, 8WC7, 8WC8, 8WCA, 8WG8, 8WRB, 8WSS, 8WW2, 8XBE, 8XBH, 8XZF, 8XZG, 8XZH, 8XZI, 8XZJ, 8YKV, 8YKW, 8YKX, 8YUU, 8YUV, 9AVG, 9AYF
KEGG IDhsa:2770
NCBI Gene ID2770
General References
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Associated Data

Drug Relations
DrugDrug groupPharmacological action?TypeActionsDetails
Guanosine-5'-DiphosphateexperimentalunknowntargetDetails
Tetrafluoroaluminate IonexperimentalunknowntargetDetails