Bifunctional enzyme IspD/IspF
Details
- Name
- Bifunctional enzyme IspD/IspF
- Synonyms
- 2.7.7.60
- MECDP-synthase
- Gene Name
- ispDF
- Organism
- Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
- Amino acid sequence
>lcl|BSEQ0011247|Bifunctional enzyme IspD/IspF MSEMSLIMLAAGNSTRFNTKVKKQFLRLGNDPLWLYATKNLSSFYPFKKIVVTSSNITYM KKFTKNYEFIEGGDTRAESLKKALELIDSEFVMVSDVARVLVSKNLFDRLIENLDKADCI TPALKVADTTLFDNEALQREKIKLIQTPQISKTKLLKKALDQNLEFTDDSTAIAAMGGKI WFVEGEENARKLTFKEDLKKLDLPTPSFEIFTGNGFDVHEFGENRPLLLAGVQIHPTMGL KAHSDGDVLAHSLTDAILGAAGLGDIGELYPDTDMKFKNANSMELLKQAYDKVREIGFEL INIDICVMAQSPKLKDFKQAMQSNIAHTLDLDEFRINVKATTTEKLGFIGRKEGMAVLSS VNLKYFDWTRL
- Number of residues
- 371
- Molecular Weight
- 41689.74
- Theoretical pI
- 6.7
- GO Classification
- Functions2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity / 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity / metal ion bindingProcessesisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway / terpenoid biosynthetic process
- General Function
- Metal ion binding
- Specific Function
- Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF).
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0011248|Bifunctional enzyme IspD/IspF (ispDF) ATGAGTGAAATGAGCCTCATTATGTTAGCAGCTGGAAATTCTACTCGTTTTAATACAAAG GTAAAAAAACAATTTTTACGACTTGGAAATGACCCTCTTTGGCTTTATGCAACAAAAAAT TTAAGCTCTTTTTATCCTTTTAAAAAAATAGTTGTTACCTCTAGTAATATCACTTATATG AAAAAATTTACCAAAAACTATGAATTCATAGAAGGTGGTGATACCAGAGCAGAATCCTTA AAAAAAGCCTTAGAGCTCATAGATAGCGAATTTGTTATGGTAAGTGATGTAGCAAGGGTA TTGGTGAGTAAAAATTTGTTTGATCGTTTGATTGAAAATTTAGATAAAGCAGATTGTATT ACCCCTGCTTTAAAAGTTGCAGATACAACACTTTTTGACAATGAAGCCTTGCAAAGAGAA AAAATCAAACTCATTCAAACCCCGCAAATTTCTAAAACCAAATTGCTTAAAAAAGCCTTG GATCAAAACCTAGAATTTACAGATGATAGCACAGCTATAGCTGCAATGGGTGGAAAAATT TGGTTTGTTGAAGGTGAAGAAAATGCAAGAAAACTAACCTTTAAAGAAGATCTTAAAAAA CTTGATTTGCCTACACCTAGCTTTGAAATTTTTACTGGCAATGGTTTTGATGTGCATGAA TTTGGAGAGAATCGCCCTTTGCTTTTAGCAGGAGTGCAAATTCATCCTACTATGGGGCTT AAAGCACACAGCGATGGAGATGTTTTAGCTCACTCTTTAACCGATGCGATTTTAGGTGCT GCAGGACTTGGAGATATAGGAGAGCTTTATCCTGATACTGATATGAAATTTAAAAATGCC AACTCCATGGAGCTTTTAAAACAAGCTTATGATAAAGTCAGAGAAATAGGTTTTGAACTC ATTAATATAGACATTTGCGTCATGGCACAAAGCCCTAAACTTAAAGACTTTAAACAAGCT ATGCAAAGCAATATAGCTCATACTTTAGATTTAGATGAATTTAGAATCAATGTTAAAGCC ACAACAACGGAAAAATTGGGTTTTATTGGAAGAAAAGAAGGTATGGCGGTGCTTAGTAGT GTAAATTTAAAATATTTTGATTGGACAAGATTATGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q9PM68 UniProtKB Entry Name ISPDF_CAMJE GenBank Protein ID 112360903 GenBank Gene ID AL111168 - General References
- Parkhill J, Wren BW, Mungall K, Ketley JM, Churcher C, Basham D, Chillingworth T, Davies RM, Feltwell T, Holroyd S, Jagels K, Karlyshev AV, Moule S, Pallen MJ, Penn CW, Quail MA, Rajandream MA, Rutherford KM, van Vliet AH, Whitehead S, Barrell BG: The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences. Nature. 2000 Feb 10;403(6770):665-8. [Article]
- Gabrielsen M, Rohdich F, Eisenreich W, Grawert T, Hecht S, Bacher A, Hunter WN: Biosynthesis of isoprenoids: a bifunctional IspDF enzyme from Campylobacter jejuni. Eur J Biochem. 2004 Jul;271(14):3028-35. [Article]