Enzymatic degradation of hybrid iota-/nu-carrageenan by Alteromonas fortis iota-carrageenase.
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Jouanneau D, Boulenguer P, Mazoyer J, Helbert W
Enzymatic degradation of hybrid iota-/nu-carrageenan by Alteromonas fortis iota-carrageenase.
Carbohydr Res. 2010 May 7;345(7):934-40. doi: 10.1016/j.carres.2010.02.014. Epub 2010 Feb 19.
- PubMed ID
- 20227066 [ View in PubMed]
- Abstract
Hybrid iota-/nu-carrageenan was water-extracted from Eucheuma denticulatum and incubated with Alteromonas fortis iota-carrageenase. The degradation products were then separated by anion-exchange chromatography. The three most abundant fractions of hybrid iota-/nu-carrageenan oligosaccharides were purified and their structures were analyzed by NMR. The smallest hybrid was an octasaccharide with a iota-iota-nu-iota structure. The second fraction was composed of two decasaccharides with iota-iota-iota-nu-iota and iota-[iota/nu]-iota-iota structures. The third fraction was a mixture of dodecasaccharides which contained at least a iota-iota-iota-iota-nu-iota oligosaccharide. The carbon and proton NMR spectra of the octasaccharides were completely assigned, thereby completely attributing the nu-carrabiose moiety for the first time.