Molecular cloning of the ecotin gene in Escherichia coli.
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Lee HR, Seo JH, Kim OM, Lee CS, Suh SW, Hong YM, Tanaka K, Ichihara A, Ha DB, Chung CH
Molecular cloning of the ecotin gene in Escherichia coli.
FEBS Lett. 1991 Aug 5;287(1-2):53-6.
- PubMed ID
- 1879537 [ View in PubMed]
- Abstract
The nucleotide sequence of a 876 bp region in E. coli chromosome that encodes Ecotin was determined. The proposed coding sequence for Ecotin is 486 nucleotides long, which would encode a protein consisting of 162 amino acids with a calculated molecular weight of 18,192 Da. The deduced primary sequence of Ecotin includes a 20-residue signal sequence, cleavage of which would give rise to a mature protein with a molecular weight of 16,099 Da. Ecotin does not contain any consensus reactive site sequences of known serine protease inhibitor families, suggesting that Ecotin is a novel inhibitor.