Heterologous expression, immunochemical and computational analysis of recombinant human interferon alpha 2b.
Article Details
- CitationCopy to clipboard
Gull I, Samra ZQ, Aslam MS, Athar MA
Heterologous expression, immunochemical and computational analysis of recombinant human interferon alpha 2b.
Springerplus. 2013 Jun 15;2(1):264. doi: 10.1186/2193-1801-2-264. Print 2013 Dec.
- PubMed ID
- 23875128 [ View in PubMed]
- Abstract
Interferon alpha 2b (IFNalpha-2b) is an important cytokine and used for antiviral and anticancer treatment. The low cost production of IFNalpha-2b with high biological activity is necessary to provide the interferon therapy to the hepatitis patients in Pakistan. In the present study, human interferon alpha 2b (hIFNalpha-2b) gene from a healthy person was cloned and overexpressed in E. coli BL21(DE3). The molecular weight of the expressed hIFNalpha-2b is 19 kDa. The over expressed recombinant hIFNalpha-2b was checked by ELISA using antibodies raised against commercially available hIFNalpha-2b. The biocomputational analysis of recombinant hIFNalpha-2b gene showed the 99.9% nucleotide sequence and 100% deduced amino acid sequence homology with reported sequences of IFNalpha-2b. The predicted 3D-structure showed mainly five alpha-helices, one 310 helix and two disulfide bonds at Cys1-Cys98 and Cys129-Cys138. The amino acid sequence alignment indicated that the disulfide linkage position is conserved in all IFNalpha family members. On the basis of sequence homology among interferon alpha family, new potent variants of hIFNalpha-2b with enhance efficacy can be produced. Indigenous production of IFNalpha-2b from gene of local population will reduce the cost and increase tolerability of interferon therapy.