Minocycline inhibits 5-lipoxygenase activation and brain inflammation after focal cerebral ischemia in rats.

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Chu LS, Fang SH, Zhou Y, Yu GL, Wang ML, Zhang WP, Wei EQ

Minocycline inhibits 5-lipoxygenase activation and brain inflammation after focal cerebral ischemia in rats.

Acta Pharmacol Sin. 2007 Jun;28(6):763-72.

PubMed ID

17506934 [ View in PubMed

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Abstract

AIM: To determine whether the anti-inflammatory effect of minocycline on postischemic brain injury is mediated by the inhibition of 5-lipoxygenase (5-LOX) expression and enzymatic activation in rats. METHODS: Focal cerebral ischemia was induced for 30 min with middle cerebral artery occlusion, followed by reperfusion. The ischemic injuries, endogenous IgG exudation, the accumulation of neutrophils and macrophage/microglia, and 5-LOX mRNA expression were determined 72 h after reperfusion. 5-LOX metabolites (leukotriene B4 and cysteinyl leukotrienes) were measured 3 h after reperfusion. RESULTS: Minocycline (22.5 and 45 mg/kg, ip, for 3 d) attenuated ischemic injuries, IgG exudation, and the accumulation of neutrophils and macrophage/microglia 72 h after reperfusion. It also inhibited 5-LOX expression 72 h after reperfusion and the production of leukotrienes 3 h after reperfusion. CONCLUSION: Minocycline inhibited postischemic brain inflammation, which might be partly mediated by the inhibition of 5-LOX expression and enzymatic activation.

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Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Minocycline	Arachidonate 5-lipoxygenase	Protein	Humans	Unknown	Inhibitor	Details